Table 1 The primers used for amplification of the capsular genes and the PCR conditions
From: Capsular gene distribution and RAPD typing of Streptococcus agalactiae isolated from pregnant women
Genes | Primer sequences 5′ to 3′ | PCR product size (bp) | Initial denaturation | Denaturation | Annealing | Extension | Final Extension | References |
|---|---|---|---|---|---|---|---|---|
cpsIa | GGTCAGACTGGATTAATGGTATGC | 521 | 95 °C for 5 min | 95 °C for 30 s | 45 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | (Yao et al. 2013) |
GTAGAAATAGCCTATATACGTTGAATGC | ||||||||
cpsIb | TAAACGAGAATGGAATATCACAAACC | 770 | 95 °C for 5 min | 95 °C for 30 s | 44 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | (Yao et al. 2013) |
GAATAAACTTCAATCCCTAAACAATATCG | ||||||||
cpsII | GCTTCAGTAAGTATTGTAAGACGATAG | 397 | 95 °C for 5 min | 95 °C for 30 s | 55 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | (Yao et al. 2013) |
TTCTCTAGGAAATCAAATAATTCTATAGGG | ||||||||
cpsIII | TCCGTACTACAACAGACTCATCC | 1826 | 95 °C for 5 min | 95 °C for 30 s | 55 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | (Yao et al. 2013) |
AGTAACCGTCCATACATTCTATAAGC | ||||||||
cpsV | GAGGCCAATCAGTTGCACGTAA | 701 | 95 °C for 5 min | 95 °C for 30 s | 54 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | (Yao et al. 2013) |
AACCTTCTCCTTCACACACTAATCCT |