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AMB Express

Table 1 LC50 value of wild-type Cry2Ab and its variants against P. xylostella larvae

From: Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity

Cry2Ab

LC50 (µg/cm2)

95% confidence level (µg/cm2)

Relatively LC50

Slope ± SEa

Wild-type

0.267

0.135–0.487

100%

0.831 ± 0.041

S145C

0.666*

0.398–1.136

40.1%

0.858 ± 0.041

S145D

0.902*

0.436–2.065

29.6%

0.502 ± 0.036

S145F

0.398

0.276–0.567

67.1%

0.798 ± 0.040

S145G

0.278

0.176–0.427

96.0%

0.922 ± 0.043

S145H

0.678*

0.481–0.960

39.4%

0.965 ± 0.043

S145I

1.376*

0.801–2.588

19.4%

0.592 ± 0.037

S145L

0.206

0.126–0.322

129.6%

1.155 ± 0.051

S145N

0.299

0.189–0.458

89.3%

0.784 ± 0.04

S145R

0.314

0.189–0.503

85.0%

0.835 ± 0.041

S145V

0.425

0.297–0.606

62.8%

0.933 ± 0.043

S145Y

0.261

0.138–0.458

102.3%

0.775 ± 0.04

N151C

17.469*

no avail

1.5%

0.091 ± 0.033

N151D

0.653*

0.409–1.057

40.9%

1.023 ± 0.044

N151F

0.375

0.219–0.622

71.2%

0.797 ± 0.040

N151G

0.697*

0.447–1.098

38.3%

0.736 ± 0.039

N151H

0.167*

0.090–0.262

159.9%

0.778 ± 0.041

N151I

0.769*

0.491–1.225

34.7%

0.885 ± 0.041

N151L

0.476

0.290–0.777

56.1%

0.894 ± 0.042

N151R

0.482*

0.322–0.718

55.4%

0.743 ± 0.039

N151S

0.691*

0.461–1.041

38.6%

0.886 ± 0.041

N151V

0.214

0.121–0.355

124.8%

0.827 ± 0.042

N151Y

0.353

0.198–0.607

75.6%

0.671 ± 0.038

T152G

0.589*

0.315–1.275

45.3%

0.913 ± 0.173

T152H

0.351

0.199–0.639

76.1%

1.058 ± 0.181

T152I

0.537*

0.323–0.962

49.7%

1.190 ± 0.194

T152L

0.433

0.228–0.897

61.7%

0.900 ± 0.170

T152R

0.514*

0.287–1.011

51.9%

1.003 ± 0.177

T152S

0.486

0.255–1.038

54.9%

0.885 ± 0.171

T152V

0.442

0.257–0.806

60.4%

1.104 ± 0.185

F157G

0.313

0.127–0.783

85.3%

0.675 ± 0.160

F157H

0.386

0.204–0.764

69.2%

0.925 ± 0.173

F157L

0.407

0.191–0.959

65.6%

0.759 ± 0.164

F157N

0.372

0.217–0.670

71.8%

1.107 ± 0.185

F157Y

0.568*

0.270–1.454

47.0%

1.319 ± 0.201

L183C

0.478*

0.196–1.496

55.9%

1.183 ± 0.192

L183D

0.612*

0.305–1.461

43.6%

1.241 ± 0.198

L183F

0.451

0.285–0.743

59.2%

1.384 ± 0.209

L183G

0.543*

0.328–0.968

49.2%

1.211 ± 0.193

L183H

0.335

0.136–0.890

79.7%

1.119 ± 0.187

L183I

0.129*

0.052–0.278

207.0%

1.357 ± 0.220

L183N

1.175*

0.374–14.473

22.7%

1.572 ± 0.234

L183R

0.688*

0.299–2.116

38.8%

1.434 ± 0.217

L183S

0.485

0.307–0.806

55.1%

1.385 ± 0.211

L183V

0.489

0.287–0.888

54.6%

1.125 ± 0.187

L183Y

0.412

0.231–0.775

64.8%

1.025 ± 0.178

L185D

0.625*

0.309–1.519

42.7%

1.501 ± 0.228

L185G

0.365

0.190–0.731

73.2%

1.512 ± 0.226

L185H

>200*

no avail

<0.1%

0.222 ± 0.191

L185I

0.756*

0.326–2.526

35.3%

1.266 ± 0.209

L185R

0.560*

0.263–1.408

47.7%

1.444 ± 0.220

L185S

0.468

0.286–0.803

57.1%

1.249 ± 0.200

L185V

0.467

0.281–0.818

57.2%

1.198 ± 0.192

I188F

0.702*

0.385–1.511

38.0%

0.961 ± 0.176

I188G

0.638*

0.422–1.006

41.8%

1.651 ± 0.246

I188H

0.372

0.206–0.692

71.8%

1.013 ± 0.179

I188L

0.715*

0.423–1.339

37.3%

1.150 ± 0.194

I188R

0.380

0.205–0.735

70.3%

0.961 ± 0.175

I188S

0.409

0.214–0.835

65.3%

0.900 ± 0.170

  1. *p < 0.05
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