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Table 2 Oligonucleotides used in this study

From: Engineering Bacillus subtilis J46 for efficient utilization of galactose through adaptive laboratory evolution

Primer name

Sequences (5′ to 3′)

araR_crRNA_F

TAAACAAACAATTAACCCCGTATTTCAAATAAAACGAAAGGCTCAGTCGAAAG

araR_crRNA_R

ATACGGGGTTAATTGTTTGTTTACACGCTCCCGGTGCGCCTGTAACATTTATTGTACAACACGA

araR_HR_A_F

TAGGCGTATCACGAGGCCCTTTCGTCGTGCAAGGCGGAGGCACCTTTGT

araR_HR_A_R

CGGATGAACGGATTTATACAGGCGCGCCGGGAGCGTGAGTTGTTTCCTTC

araR_HR_B_F

GAAGGAAACAACTCACGCTCCCGGCGCGCCTGTATAAATCCGTTCATCCG

araR_HR_B_R

TTTGTATTGTGTTGAAATATGTTTTGTTATTCATTCAGTTTTCGTGCGGACTG

araR_HR_del_A_F

AATAGGCGTATCACGAGGCCCTTTCGTATGTTACCAAAATACGCGCAAGTAAA

araR_HR_del_A_R

TCCGTTGTAAATGTCACGATCATATCCGGAGGCAAAAGGAATGCCGTTTTTCTCC

araR_HR_del_B_F

GGAGAAAAACGGCATTCCTTTTGCCTCCGGATATGATCGTGACATTTACAACGGA

araR_HR_del_B_R

TTTGTATTGTGTTGAAATATGTTTTGTTATTCATTCAGTTTTCGTGCGGACTG

glcR_crRNA_F

CGCTTCACCATGCCATCCTCTATTTCAAATAAAACGAAAGGCTCAGTCGAAAG

glcR_crRNA_R

AGAGGATGGCATGGTGAAGCGCACGCTCCCGGTGCGCCTGTAACATTTATTGTACAACACGA

glcR _HR_A_F

TAGGCGTATCACGAGGCCCTTTCGTAATTACAACGGAGCAGATCTGTACG

glcR _HR_A_R

TTTTCCGCTTCACCATGCCATCCTCTTCATGCGCAAATTGTAATGCCATG

glcR _HR_B_F

CATGGCATTACAATTTGCGCATGAAGAGGATGGCATGGTGAAGCGGAAAA

glcR _HR_B_R

TTGTATTGTGTTGAAATATGTTTTGTCAGTCCTTTCCTTCATCCTGCTC

sigA_RT_F

GGCAGAGAACCAACACCTGA

sigA_RT_R

TCACCAAGGTGCGAGTCATC

araE_RT_F

CTTTGTCGCTTCACGCTCTG

araE_RT_R

TCCTCTGATGATGCTCGGGA

araR_RT_F

CTTTGTCGCTTCACGCTCTG

araR_RT_R

TCCTCTGATGATGCTCGGGA

araB_RT_F

TTGGCATTACAGAGCCAGGG

araB_RT_R

CGGGAGAATTCCGTTGTCCA

araD_RT_F

CCTTGATGGAGAGGTCGTCG

araD_RT_R

AGCTTGTCGCCCATTGAGAA

araL_RT_F

ATGATTGGGGCGATAGAGGC

araL_RT_R

ATGTGCGGACAGTCCCATTG

araM_RT_F

CTTGACGCCTGTATCGCACA

araM_RT_R

AAATATGATGCTCGCCGCCT

araN_RT_F

TTGTGCCGAAACAAGCCAAG

araN_RT_R

CTCCAGACATCCCAGCGAAG

abnA_RT_F

TCGGGCAATGACCAATGGAA

abnA_RT_R

AGCCCGAGCTCCAATTCAAA

galE_RT_F

TCCGACAAAAGACGGGACAG

galE_RT_R

TGTGCCTGTTCCAAGGTTGT

galT_RT_F

AGTGAAGTGGCCGATGTCTG

galT_RT_R

ATTATGGGGCGTATCGCCTG

galK_RT_F

CTATAACGGCGGGCATGTCT

galK_RT_R

TCCTTGATGCCTGCGTTTCT