Table 1 Bacterial strains and plasmid used in this study

E. coli

DH5α

F^– φ80lacZΔM15 Δ(lacZYAargF)U169 recA1 endA1 hsdR17(r_K^–, m_K⁺) phoA supE44 λ^–thi-1 gyrA96 relA1; Cloning host

Invitrogen

HST04

F⁻, ara, Δ(lac-proAB)[Φ80dlacZΔM15], rpsL(str), thi, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, dam, dcm

Takara

B. subtilis

J46

KCCM12388P

This study

BSGA14

B. subtilis J46 adaptively evolved in M9 medium with galactose as a sole carbon source, KACC92527P

This study

BSGALE1

J46 araR^H226R

This study

BSGALE2

J46 Δ araR

This study

BSGALE3

J46 Δ glcR

This study

BSGALE4

J46 araR^H226R; Δ glcR

This study

Plasmids

pHTCpf1

pHT01 derivative plasmid, containing Pgrac-Cpf1, Amp^R, Km^R

Addgene

pADsacA

pAD123 derivative, containing sacA targeting 23 bp crRNA transcription module and 1.2 kb donor DNA, Amp^R, Cm^R

Addgene

pAD-araR

pADsacA derivative, araR cRNA, Amp^R, Cm^R

This study

pAD-araRHR

pAD-araR derivative, araR cRNA, homologous arm of araR, Amp^R, Cm^R

This study

pAD-araRdelHR

pAD-araR derivative, araR cRNA, homologous arm of araR for deletion, Amp^R, Cm^R

This study

pAD-glcR

pADsacA derivative, glcR cRNA, Amp^R, Cm^R

This study

pAD-glcRHR

pAD-glcRHR derivative, glcR cRNA, homologous arm of araR, Amp^R, Cm^R

This study