Fig. 4

Reverse engineering of B. subtilis J46 introduced mutation of ΔglcR. a The growth profile and galactose consumption of B. subtilis J46, BSGA14, BSGALE3 and BSGALE4 in M9 minimal medium containing 2% (w/v) galactose. Black and white symbols mean optical density at 600 nm and galactose. Triangles, squares, circles, and diamonds represent samples from B. subtilis J46, BSGA14, BSGALE1 and BSGALE4 respectively. The standard deviation estimated from triplets is represented by the error bars. b Change in gene expression levels of the galactose metabolic pathways. Log₂ fold change compared to genes related to galactose catabolism of the parental strain