Improving the thermostability of Pseudoalteromonas Porphyrae κ-carrageenase by rational design and MD simulation

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Table 2 Property comparisons of S190R and WT

	^aRelative enzyme activity (%)	^bT_opt (°C)	^cpH_opt	T₅₀³⁰ (°C)	T_m (°C)	K_m (mg/mL)	V_max (U/mg)
WT	100	40	8.0	49.7	64.4	2.87	625
S190R	98.1	50	8.0	52.0	66.2	7.00	1250

Notes: ^aEnzyme activity was measured using the standard measurement method described in “Materials and methods”, and the enzyme activity of WT was defined as 100%. ^bT_opt standed for the optimal temperature. ^cpH_opt standed for the optimal pH.