Fig. 5

Differentiation of L. crispatus and L. jensenii cells by colony immunoblots and fluorescence microscopy. A L. crispatus 33820 and L. jensenii 115-3-CHN mixture incubated with Lc58RFP and Lj75GFP and imaged as indicated. The images are representative of two independent experiments. B Colony immunoblot of a L. crispatus 33280 and L. jensenii 115-3-CHN mixture performed as described in “Materials and methods”. An image of the plate prior to colony lift (L), a fluorescence multiplex image of the colony immunoblot (M), and an enlarged image of a portion of the blot are shown. The images are representative of three independent experiments