Fig. 4
From: Nanobodies as potential tools for microbiological testing of live biotherapeutic products
Characterization of antigen display. Binding of Lactobacillus by Lc58 and Lj75 was assessed at different growth phases by flow cytometry and visualized by fluorescence microscopy. A Exponential and B stationary phase L. jensenii 115-3-CHN cells labeled with Lj75BFP. C Percentage of exponential or stationary phase-grown L. crispatus or L. jensenii bound by Lc58RFP or Lj75BFP, respectively, by flow cytometry. Data points represent the mean ± standard deviation from six biological replicates in two independent experiments. D Unlabeled L. crispatus control (left) and L. crispatus 33820 labeled with Lc58RFP (right), shown with fluorescence only (top) and merged brightfield and fluorescence (bottom). E Unlabeled L. jensenii 115-3-CHN (left) and L. jensenii 115-3-CHN labeled with Lj75GFP (right) shown with fluorescence only (top) and merged brightfield and fluorescence (bottom)