Fig. 3

Identification of antigens bound by Lc58 and Lj75 and culture conditions under which they were detected. A An approximately 55-kDa band was detected in the L. crispatus 125-2-CHN lysate (lane 1) and in the E. coli lysate containing the plasmid expressing L. crispatus 125-2-CHN S-layer protein, EEU18441.1 (lane 3), when probed with Lc58. No bands were observed in the E. coli lysates from cells containing the empty vector (lane 2), the plasmid expressing Bacterial Ig-like domain protein, EEU19392.1 (lane 4), or the plasmid expressing cell separation protein, EEU18637.1 (lane 5). B An approximately 70-KDa band was observed in the L. jensenii JV-V16 lysate (lane 1) and in the E. coli lysate containing the plasmid expressing NlpC/P60 protein, EFH30000.1 (lane 3), when probed with Lj75. No bands were observed in the E. coli lysates from cells containing empty vector (lane 2) or the plasmid expressing the uncharacterized protein, EFH30544.1 (lane 4). C When probed with Lj75, an approximately 80-kDa band was observed in the L. jensenii 115-3-CHN lysate (lane 1) and in the E. coli lysate containing the plasmid expressing the full length NlpC/P60 protein EEX23860.1 (lane 2)