Fig. 2

Binding specificity of nanobodies by flow cytometry. Lactobacillus strains were incubated with Lj94, Lc38, Lc58RFP, or Lj75BFP and analyzed by flow cytometry. L. crispatus EX8 cells were incubated with Dylight alone (A) or Dylight plus 10 µg/ml Lc38 (B). L. jensenii JV-V16 cells were incubated with Dylight alone (C) or Dylight plus 10 µg/ml Lj94 (D). L. crispatus 33820 cells (E) or incubated with 10 µg/ml Lc58 (F). L. jensenii 115-3-CHN cells (G) or incubated with 40 µg/ml Lj75BFP (H). This experiment was repeated twice with similar results