Fig. 1

Binding specificity of nanobodies by colony immunoblots. Lactobacillus strains were patched or spotted onto MRS agar and transferred to nitrocellulose filters, which were probed with Lj94, Lj75, Lc38, or Lc58 as described in “Materials and methods”. A Blot of L. crispatus strains probed by buffer control (top) or Lc38 (middle) and a strain key (bottom) with corresponding table (right) identifying the location of each L. crispatus strain on the blot. B Summary results of immunoblot experiments with Lc58, Lc38, Lj75, and Lj94