Table 2 Oligonucleotides used in this study
From: Unveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa
Primer | Sequence 5′–3′ | Application |
|---|---|---|
pccg XbaI gh3-3 FW | aaaaTCTAGAATGAAGTTCGCCATTCCGCT | Cloning in pccg-1 plasmid |
pccg PacI gh3-3 RV | aaaaTTAATTAATCAGGGAAGAACCTCCTCGAG | Cloning in pccg-1 plasmid |
gh3-3 native FW part2 | TCTCGAGGAGGTTCTTCCCTGAggcggaggcttaatcggctt | Cloning in plasmid with native promoter (pN) |
AQUA_AmpR_ColE1_pYTK095_RV | acggttatccacagaatcaggg | Cloning in pN plasmid |
AQUA_cPCR2_fw | ctgcgttatcccctgattctgtg | Cloning in pN plasmid |
native gh3-3 RV part1 | GGCCCAATGGGACTTGGCATCCTgatggactgctccttctagcg | Cloning in pN plasmid |
native gh3-3 | AGGATGCCAAGTCCCATTGGGCC | Cloning in pN plasmid |
native gh3-3 RV part2 | TCAGGGAAGAACCTCCTCGAG | Cloning in pN plasmid |
pccg native gh3-3 sequencing primer 1 | CCGATGCCGAACATCTGTTCA | Sequencing of gluc-1 |
Tail-hph3 | CGACAGACGTCGCGGTGAGTTCAG | Genotyping |
gh3-3 FW | CTGTTACGGCGGATATCACCAACACG | Genotyping |
gh3-3 RV | AAACGAGCCCAGTTGACTCCACATGC | Genotyping |
gh3-3 genotyping checking primer FW | GGGATGTGAGTTCATGGGATACGG | Genotyping |
p492_his3_check_R | GTCAGCATCCGTCTTGAGCAC | Genotyping |