Fig. 6
From: Unveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa
Overview comparison of domain 2 between the GH3-3 crystal structure and the Gluc-1 model. A. Domain 2 in GH3-3 and Gluc-1 alignment. The Gluc-1 model calculated from SWISS-MODEL (Waterhouse et al. 2018) was aligned to GH3-3 crystal structure (5nbs.1.A) (Karkehabadi et al. 2018). The α/β region of domain 2 is colored in blue. Loops III and IV are colored in green and yellow, respectively. Red color indicates mismatched positions between the two structures. The L425P point mutation is highlighted in orange (black box). B. Mutated position in the alignment between GH3-3 and Gluc-1. The structure in the L425P region in the structural alignment between GH3-3 and Gluc-1 was compared. The hydrogen bond between L425 and L600 in GH3-3 is colored in yellow within 2.8 Å