Fig. 4

Growth assays of β-glucosidase mutant strains in plates and in liquid cultures. A. Growth and morphology on cellobiose plates. The same amount of conidia was spotted on VMM + 1% sugar + 0.11 M Tris (pH 8) plates and incubated at 25° C for 7 days. Tris was added to achieve colonial growth (acc. to Huberman et al. 2017). These are representative pictures from one of three independent experiments. Magnified zoom-in images of inlets are shown in the last row. Scale bars represent 10 mm. B. and C. Growth of β-glucosidase mutant strains in liquid medium. Conidia were inoculated into liquid VMM + sucrose, cellobiose (B) or CMC (C). Three biological replicates for each strain were used. Data are means ± standard error. Asterisks indicate statistically significant differences at p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***) relative to wild-type strain, unless otherwise indicated with brackets