Fig. 1
From: Unveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa
Schematic of the mutation in the gluc-1 mutant and conservation analysis. A. Sequencing analysis revealed that gluc-1 mutant has a mutation in NCU08755, a member of the beta-glucosidase family 3 of glycoside hydrolases. The gluc-1 mutation at nucleotide residue 1274 leads to a substitution of leucine for proline at amino acid residue 425. The nucleotide area surrounding the gluc-1 mutation is shown for the wild-type (WT, top) and the mutant (bottom) alleles. The encoded amino acid sequence is shown below in grey. The genomic DNA is represented below, with introns in orange. B. Alignment of GH3-3 and other closely related β-glucosidases of various filamentous fungi. The conserved residue 425 is indicated by a red box. Organisms: Ab Agaricus bisporus, Af Aspergillus fumigatus, An Aspergillus niger, Ao Aspergillus oryzae, Po Penicillium oxalicum, Tr Trichoderma reesei, Tt, Thermothelomyces thermophilus. C. Schematic of domains in the GH3-3 amino acid sequence (acc. to Karkehabadi et al. 2018). Domains 1 and 3 are depicted in light grey. The α/β region of domain 2 is colored in blue. Loops III and IV are colored in green and yellow, respectively. Amino acid residue 425 is shown in red and indicated by an arrow