Anti-leishmanial effects of Eryngium planum and Ecbilliun elaterum methanolic extract against Leishmania major

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Table 1 Composition (expressed in %) of the methanolic extracts of E. planum and E. elaterum using gass chromatography and mass spectrophotometry assay

Component	R	E. planum	E. elaterum	Identification	CAS No.
α-Pinene	945	11.05 ± 0.07	10.24 ± 0.021	RI, MS, Co-GC	1254-03-2
β-Pinene	958	2.65 ± 0.043	1.05 ± 0.02	RI, MS	1152-13-5
Myrcene	962	3.14 ± 0.07	4.94 ± 0.033	RI, MS	982-32-4
Limonene	974	0.81 ± 0.014	–	RI, MS	982-31-6
(Z)-β‐Ocimene	978	–	0.724 ± 0.019	RI, MS	982-30-2
Terpinolene	1021	1.035 ± 0.034	0.586 ± 0.025	RI, MS	215-14-2
Camphor	1125	5.05 ± 0.17	3.3 ± 0.047	RI, MS	215-32-1
Terpinen-4‐ol	1087	0.92 ± 0.024	0.51 ± 0.078	RI, MS	119-25-3
Myrtenol	1134	1.34 ± 0.064	0.55 ± 0.035	RI, MS	630-02-1
Linalyl acetate	1052	0.92 ± 0.032	0.28 ± 0.015	RI, MS	630-02-3
α-Terpenyl acetate	1134	0.023 ± 0.032	0.54 ± 0.022	RI, MS, Co-GC	630-02-8
β-Bourbonene	1066	2.31 ± 0.54	0.82 ± 0.034	RI, MS	6845-62-2
β-Caryophyllene	1087	12.54 ± 0.97	4.15 ± 0.67	RI, MS	11495-03-5
β -Copaene	1199	0.4 ± 0.044	0.84 ± 0.027	RI, MS	4856-05-1
(Z)-β‐Farnesene	1256	0.4 ± 0.004	–	RI, MS	13452-21-2
Germacrene D	1291	3.51 ± 0.054	9.74 ± 0.058	RI, MS	4125-05-4
Bicyclogermacrene	1341	8.45 ± 0.11	3.61 ± 0.087	RI, MS	3215-02-5
Caryophyllene oxide	1491	14.35 ± 0.94	19.54 ± 1.34	RI, MS	3215-41-3
γ-Eudesmol	1585	4.5 ± 0.054	6.1 ± 0.071	RI, MS, Co-GC	483-74-2
α-Bisabolol	1550	7.9 ± 0.92	8.1 ± 0.054	RI, MS, Co-GC	515-71-8
Thymol	1310	1.3 ± 0.021	0.9 ± 0.011	RI, MS, Co-GC	87-21-3
Carvacrol	1355	0.4 ± 0.011	0.6 ± 0.013	RI, MS	469-32-4
Eugenol	1425	0.3 ± 0.008	0.3 ± 0.011	RI, MS	458-21-8
3-Octanol acetate	1250	0.6 ± 0.014	0.2 ± 0.031	RI, MS	6750-52-5
Docosane	2200	–	0.21 ± 0.004	RI, MS	520-15-4
Nonacosane	2400	0.2 ± 0.01	0.2 ± 0.01	RI, MS	520-16-7

Note: R retention indices were defined relative to a series of n-alkanes (C8–C40) on capillary column VF5‐MS, RI identification made using the literature [Dario Kremer, 2021], MS identification made with help of database NIST02, Wiley 7 and homemade library, Co–GC identification using reference compounds, CAS No. CAS Registry Number [Dario Kremer, 2021]