Fig. 4
Preparation and characterization of PCV3 Cap VLPs. a Expression of full-length PCV3 Cap with mutated NLSP (Cap/BacNLSPm) recombinant protein in Sf9 cells. Sf9 cells were infected with the recombinant baculovirus and fixed at 48 h post-infection, followed by immunofluorescence assay with the MAb CCC160. b The purified Cap/BacNLSPm VLPs were negatively stained (b) and further recognized with the MAb CCC160 by immunogold staining (c), followed by observation using a TEM. The scale bar is 100 nm