Table 2 Amplified fragment size, primer, and probe sequences of CCOL2A1 and β-actin determined using qPCR from in vivo expression identification
From: Transformed Salmonella typhimurium SL7207/pcDNA-CCOL2A1 as an orally administered DNA vaccine
Gene | Primer/Probe | Sequence (5′ to 3′) | Size (bp) |
|---|---|---|---|
β-actin | Mouse β-actin F-primer | ACCGTGAAAAGATGACCCAGAT | 70 |
Mouse β-actin R-primer | GCCTGGATGGCTACGTACATG | ||
Mouse β-actin probe | (FAM)TTTGAGACCTTCAACACC(MGB) | ||
CCOL2A1 | CCOL2A1 F-primer | TCTTGTTGGTCCCAGAGGTGA | 118 |
CCOL2A1 R-primer | ACCCTTGGGTCCGTCAGTG | ||
CCOL2A1 probe | (FAM) CGTGGATTCCCCGGTGAACGC(MGB) |