Fig. 3
From: Transformed Salmonella typhimurium SL7207/pcDNA-CCOL2A1 as an orally administered DNA vaccine
CCOL2A1 expression in BALB/c mouse ilea. Bacterial suspension of the transformed strain SL7207/pcDNA-CCOL2A1 (1010 CFU/mL) was administered by gavage at 4.0 × 109 CFU and 0.4 mL per mouse. M: Male. F: Female. 1 M represents male mouse No. 1, 1F represents female mouse No. 1, and so on. Standards and samples for RT-qPCR determination were run in triplicate. Differences in relative expression were evaluated using the Kruskal–Wallis H test for multiple samples. χ2 (H statistic) = 16.111, df = 5, P = 0.007. Significant differences between samples indicated individual differences in CCOL2A1 expression among mouse ilea. df: degree of freedom