Fig. 2
From: Transformed Salmonella typhimurium SL7207/pcDNA-CCOL2A1 as an orally administered DNA vaccine
Identification of PCR amplification products via sequencing. a Representative sequence alignment (top/middle) and sequencing peak (bottom) diagrams of the amplification product from the full-length sequence using PCR. b Electrophoretic identification of the amplification product from a partial sequence using PCR. 1. Blank control, ddH2O template. 2: Negative control, original extract of blank strain SL7207 as template. 3: Positive control, pcDNA-CCOL2A1 plasmid as template. 4. Marker. 5, 6, and 7. Amplification products using extracts of three single colonies from transformed SL7207 strains as templates. c Representative sequence alignment (top/middle) and sequencing peak (bottom) diagrams of the amplification product from a partial sequence using PCR. The pictures at the top, middle, and bottom in a and c are partially displayed