Table 2 The observed and predicted responses of the Box Behnken design
Run order | pH | Inoculum size (% v/v) | Incubation period (days) | Observed response | Predicted response |
|---|---|---|---|---|---|
1 | 6.5 | 1 | 1 | 0 | 2.50 |
2 | 9 | 2.5 | 1 | 16 | 15.63 |
3 | 6.5 | 2.5 | 3.5 | 18 | 19.44 |
4 | 6.5 | 2.5 | 3.5 | 19 | 19.44 |
5 | 9 | 2.5 | 6 | 0 | 0.88 |
6 | 6.5 | 2.5 | 3.5 | 20 | 19.44 |
7 | 4 | 2.5 | 6 | 20 | 19.13 |
8 | 4 | 2.5 | 1 | 16 | 13.87 |
9 | 6.5 | 4 | 1 | 0 | 0 |
10 | 6.5 | 1 | 6 | 20 | 21.25 |
11 | 9 | 1 | 3.5 | 17 | 14.82 |
12 | 6.5 | 2.5 | 3.5 | 20 | 19.44 |
13 | 6.5 | 2.5 | 3.5 | 19 | 19.44 |
14 | 4 | 1 | 3.5 | 24 | 23.07 |
15 | 9 | 4 | 3.5 | 16 | 15.82 |
16 | 6.5 | 4 | 6 | 0 | 1.25 |
17 | 4 | 4 | 3.5 | 22 | 24.07 |
\(\begin{aligned} {\text{CPC activity}}\; = & \; - \;{26}.{81167}\; + \;{1}.{15}000 \;*{\text{A}}\; + \;{11}.{3}0000\;*{\text{B}}\; + \;{2}0.00{111} \;*{\text{C}}\; \\ - & \;0.{8}0000\;*{\text{A}}\;*{\text{C}}\; - \;{3}.{13333}\;*{\text{B}}\;*{\text{ C}}\; - \;{1}.{13111}\;*{\text{C2}} \\ \end{aligned}\) (2) | |||||