Table 2 Primers and probes for RPA-LFD assays

S. aureus

Nuc

LFD-Sau-F3

AATTACATAAAGAACCTGCGACTTTAATTA

LFD-Sau-R2

[biotin] ATGCACTTGCTTCAGGACCATATTTCTCTA

NFO-Sau

[FAM]GCGATTGATGGTGATACGGTTAAATTAATG[THF]ACAAAGGTCAACCA[C3spacer]

K.peneumoniae

CelB

LFD-Kpn-F2

TTACCGCCATTCTGGTGGCGATAATTTCAAC

LFD-Kpn-R3

[biotin] CAAGTGATGTTAGCGGAACCTGGATTAAT

NFO-Kpn

[FAM]AATGTCCCTGAATTTATCTCTAAATCGTTC

[THF]CTTCATTGATTCCAG[C3spacer]

P. aeruginosa

Eta

LFD-Pae-F2

CTGAACGCCGGTAACCAGCTCAGCCACATG

LFD-Pae-R3

[biotin]GGCACAACACCTTGCCGCTGGCCCATTCGC

NFO-Pae

[FAM]CGAAGCTGGCGCGCGATGCCACCTTCTTCG

[THF]CAGGGCGCACGAGAG[C3spacer]

H.influenzae

Fuck

LFD-Hin-F1

CGTCAATGCTCACTCAACGCTTAACTGGTC

LFD-Hin-R1

[biotin]GAGTATCATGTCCACAAGAAATGACAGGTAC

NFO-Hin

[5’FAM]AGCATCGCTGGGTTTAAGTAATAACCATTTCCCT[THF]CTATGCGTTATGCAGGT[C3spacer]