Table 1 Primers for Basic RPA assay
Target bacteria | Target region | Primer name | Sequence (5’- 3’) |
|---|---|---|---|
S. aureus | Nuc | Sau-F1 | AGCAAATGCATCACAAACAGATAACGGCGT |
Sau-F2 | GATCCAACAGTATATAGTGCAACTTCAACT | ||
Sau-F3 | AATTACATAAAGAACCTGCGACTTTAATTA | ||
Sau-R1 | CCTTGACGAACTAAAGCTTTGTTTACCATT | ||
Sau-R2 | ATGCACTTGCTTCAGGACCATATTTCTCTA | ||
Sau-R3 | TTCTTTGACCTTTGTCAAACTCGACTTCAA | ||
K. peneumoniae | CelB | Kpn-F1 | CGAATATTCGTGGCGATAACTCGCAAGG |
Kpn-F2 | TTACCGCCATTCTGGTGGCGATAATTTCAAC | ||
Kpn-F3 | TTCAATCCCTGGCCAATGGCTGGGGCCCAT | ||
Kpn-R1 | CGGAGATAATTGTAAACAGCGTCAGGATGG | ||
Kpn-R2 | ATGCTCCCATAACCAACGCTGGTAATGACT | ||
Kpn-R3 | CAAGTGATGTTAGCGGAACCTGGATTAAT | ||
P. aeruginosa | Eta | Pae-F1 | CGAGAAGCCTTCGAACATCAAGGTGTTCAT |
Pae-F2 | CTGAACGCCGGTAACCAGCTCAGCCACATG | ||
Pae-F3 | TCTACACCATCGAGATGGGCGACGAGTTGC | ||
Pae-R1 | ATGGCTGATGGCGAGCGTCGGCTGCATCTC | ||
Pae-R2 | GCGGCTGGGCCTGGGCCATGACCACGCTGA | ||
Pae-R3 | GGCACAACACCTTGCCGCTGGCCCATTCGC | ||
H. influenzae | Fuck | Hin-F1 | CGTCAATGCTCACTCAACGCTTAACTGGTC |
Hin-F2 | CACTACAGATCACACAATGGCGGGAACATCAAT | ||
Hin-F3 | CAATGATGACAAACCTTACTAGCGGTAATTG | ||
Hin-R1 | GAGTATCATGTCCACAAGAAATGACAGGTAC | ||
Hin-R2 | ATTCAGCCCTGCACCAGACCCAAACACAGC | ||
Hin-R3 | CATTAAGATTTCCCAGGTGCCAGAACTTAAC |