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Fig. 3 | AMB Express

Fig. 3

From: Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples

Fig. 3

Sensitivity of RPA-LFD assays. A, B, C, D and E represent the results of S. aureus, K. peneumoniae, P. aeruginosa, and H. influenzae RPA-LFD sensitivity assays, respectively. Serially diluted DNA concentration of targeted bacteria (6 × 105 CFU/mL, 6 × 104 CFU/mL, 6 × 103 CFU/mL, 6 × 102 CFU/mL, 60 CFU/mL and 6 CFU/mL per reaction) was tested by RPA-LFD assays at 38 °C for 10 min. This experiment was repeated three times for low-concentrated samples (60 CFU/mL-6 CFU/mL). NC: negative control; C: control line; T: test line

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