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Fig. 1 | AMB Express

Fig. 1

From: Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples

Fig. 1

Bacteria-spiked blood samples operation procedures using the integrated isothermal amplification system. (A) Placed rubber rings on the detection device and inserted LFD at inspection windows. (B-C) RPA reaction mixtures and buffer were added to left channel and right channel of bottom container respectively. (D) Inserted top cover into the reaction chamber and sealed with sealing films. (E) The assembled detection device was heated in a matched metal heat block at 38 °C for 10 min. (F-G) Bottom pad was pierced by top cover. Then, tilting and gently shaking the detection device. (H) Observed test results

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