Changes in physicochemical properties and microbial community succession during leaf stacking fermentation

AMB Express

Table 1 Experimental factors predicting α- and β-diversity of microbial communities in cigar tobacco leaves sourced from different locations

Facor	Sample group	α-diversity (Shannon)^a						β-diversity^b
		Fungal			Bacterial			Fungal Bray-Curtis		Bacterial weighted UniFrac
		Df	F	P	Df	F	P	R²	P	R²	P
Origin	PE	5,12	4.188	0.020	5,12	41.93	0.000	0.516	0.005	0.762	0.001
	LC	5,12	1.116	0.402	5,12	1.204	0.364	0.565	0.017	0.614	0.015
	DH	5,12	1.792	0.189	5,12	1.259	0.343	0.634	0.014	0.410	0.131
	All regions	5,48	0.098	0.992	5,48	2.082	0.084	0.071	0.071	0.101	0.383
Stage	F0	2,6	10.83	0.010	2,6	68.18	0.000	0.691	0.014	0.640	0.010
	F1	2,6	4.831	0.056	2,6	54.24	0.000	0.842	0.019	0.627	0.025
	F2	2,6	29.95	0.001	2,6	67.96	0.000	0.858	0.004	0.560	0.011
	F3	2,6	6.187	0.035	2,6	326.8	0.000	0.516	0.143	0.535	0.052
	F4	2,6	36.29	0.000	2,6	0.648	0.556	0.912	0.006	0.901	0.006
	F5	2,6	0.415	0.678	2,6	4.019	0.078	0.889	0.017	0.855	0.003
	All stages	2,51	26.9	0.000	2,51	0.0781	0.000	0.566	0.001	0.411	0.001

^a One-way ANOVA (P < 0.05). ^b Permutational multivariate analysis of variance (PERMANOVA) using distance matrices (P < 0.05). Df denotes degrees of freedom, F denotes statistical magnitude, R² denotes correlation coefficients, and P denotes significant differences at the 0.05 level