Table 1 The primers used in the present investigation were specifically developed for this research
Gene | Sequence (5′ → 3′) | TM (℃) | Size (bp) |
|---|---|---|---|
omp10 | F: 5′-ATGGAAAGTATGGATATGAAAAG-3′ | 57 | 381 |
R: 5′-TTAACCAGCATTACGACGTGTT-3′ | |||
GAPDH | F: 5′-TGTGTCCGTCGTGGATCTGA-3′ | 60 | 78 |
R: 5′-CCTGCTTCACCACCTTCTTGA-3′ | |||
IFN-γ | F:5′-AGCGGCTGACTGAACTCAGATTGTAG-3′ | 60 | 199 |
F: 5′-GTCACAGTTTTCAGCTGTATAGGG-3′ | |||
IL-10 | F: 5′-CTTGGGACTGATGCTGGTGAC-3′ | 60 | 162 |
R: 5′-TCTTTTCTCATTTCCACGATTTC-3′ | |||
IL-4 | F: 5′-CGAAGAACACCACAGAGAGTGAGCT-3′ | 60 | 180 |
R: 5′-GACTCATTCATGGTGCAGCTTATCG-3′ | |||
TNF-α | F: 5′-AGGCACTCCCCCAAAAGATG′ | 60 | 183 |
R: 5′-CCACTTGGTGGTTTGTGAGTG-3′ |