Fig. 5

Measurement of the secretion level of IFN-γ, TNF-α, IL-4 and IL-10 cytokines in the small intestine and spleen by real-time PCR method. A Expression levels of IFN-γ cytokine in spleen and small intestine before and after challenge with B. abortus, B measurement of TNF-α levels before and after challenge, C, D measurement of IL-10 and 1L-4 expression levels in spleen and small intestine before and after challenge. The data are replicated three times and averaged. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001