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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Design of an oral vaccine using Lactococcus lactis against brucellosis: an in vitro and in vivo study

Fig. 2

A Electrophoresis of the recombinant L. lactis protein mixture using SDS-PAGE. In lane 2, L. lactis bacteria were transformed with the recombinant Omp10 vector pNZ8148-Usp45. Lane 1: a vector that does not express the desired protein was used to transform L. lactis bacteria. Lane M: indicator (Thermo Fisher Scientific, USA). B To confirm the existence of recombinant protein inside L. lactis bacteria that have undergone transformation, a western blot analysis was conducted. Lane 1: marker ((Thermo Fisher Scientific, USA), Lane 2: the L. lactis bacteria were subjected to transformation using the recombinant vector pNZ8148-Omp10-Usp45. Lane 3: L. lactis bacteria were transformed using a vector that did not include the gene of interest. Lane 4: GAPDH

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