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Table 5 Kinetic analysis of ArgOX and ArgOX/E486Q for l-citrulline

From: Modification of substrate specificity of l-arginine oxidase for detection of l-citrulline

Enzyme variant

Km

kcat

kcat / Km

(mM)

(s–¹)

(s–¹mM–¹)

ArgOX

97.2 ± 3.1

2.71 ± 0.06

0.028 ± 0.001

ArgOX/E486Q

42.6 ± 2.0

6.22 ± 0.83

0.146 ± 0.013

  1. Values are presented as mean ± standard deviation