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AMB Express

Table 4 Specific activity of ArgOX and ArgOX/E486Q for l-arginine or l-citrulline and substrate specificity

From: Modification of substrate specificity of l-arginine oxidase for detection of l-citrulline

Enzyme variant

Specific activity (U/mg)a

Cit/Arg (%)b

 

l-arginine

l-citrulline

 

ArgOX

1.74 ± 0.01

0.86 ± 0.03

49.50

ArgOX/E486Q

2.17 ± 0.04

3.25 ± 0.50

150.00

  1. Values are presented as mean ± standard deviation
  2. a Specific activity was measured after incubation in 200 mM phosphate buffer (pH 6.0) with 50 mM substrate (l-arginine or l-citrulline) at 37 ℃
  3. b Cit/Arg value was calculated using the following formula:
  4. (Specific activity of l-citrulline)/(Specific activity of l-arginine) × 100
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