Table 3 Oxidase activity of ArgOX mutants for l-arginine and l-citrulline
From: Modification of substrate specificity of l-arginine oxidase for detection of l-citrulline
Enzyme activity assay | ArgOX | Mutants | |||
|---|---|---|---|---|---|
ArgOX/E486Q | ArgOX/E486N | ArgOX/E486H | ArgOX/E486R | ||
l-arginine Oxidase activity (U/mL) | 1.11 ± 0.01 | 1.73 ± 0.03 | 3.03 ± 0.05 | 1.25 ± 0.02 | 0.33 ± 0.01 |
l-citrulline Oxidase activity (U/mL) | 1.06 ± 0.01 | 1.37 ± 0.00 | 0.06 ± 0.01 | 0.11 ± 0.01 | Not detected |