Skip to main content
AMB Express

Table 2 Specific activity of oxidase or dehydrogenase activity for l-arginine or l-citrulline and substrate specificity

From: Modification of substrate specificity of l-arginine oxidase for detection of l-citrulline

Enzyme activity assay

Specific activity (U/mg)a

Cit/Arg(%)

l-arginine

l-citrulline

Oxidase activity

89.7 ± 0.8

1.07 ± 0.02

1.20

Dehydrogenase activity

1.74 ± 0.01

0.86 ± 0.03

49.50

  1. Values are presented as mean ± standard deviation
  2. a Specific activity was measured after incubation in 200 mM phosphate buffer (pH 6.0) with 50 mM substrate (l-arginine or l-citrulline) at 37 ℃
  3. b Cit/Arg value was calculated using the following formula:
  4. (Specific activity of l-citrulline)/(Specific activity of l-arginine) × 100
Back to article page