Table 1 List of primer sequences for ArgOX mutants
From: Modification of substrate specificity of l-arginine oxidase for detection of l-citrulline
Sample | Sequence | ||
|---|---|---|---|
E486H_Fw | 5’- | GGTGGCTGGCATcatTGGAAAGCGAATTAT | -3’ |
E486N_Fw | 5’- | GGTGGCTGGCATaatTGGAAAGCGAATTAT | -3’ |
E486Q_Fw | 5’- | GGTGGCTGGCATcaaTGGAAAGCGAATTAT | -3’ |
E486R_Fw | 5’- | GGTGGCTGGCATcgcTGGAAAGCGAATTAT | -3’ |
E486_Rv | 5’- | AATCAGATCCAGACGATAATTCGCTTTCCA | -3’ |