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Table 2 Experimental levels, values of variables and constant factors used in three-level factorial design

From: Potential of Y. lipolytica epoxide hydrolase for efficient production of enantiopure (R)-1,2-octanediol

Variable Factors

Coded

Levels

  

-1

0

+ 1

Substrate (mM)

A

1

8

15

Enzyme (µg)

B

75

112.5

150

Constant Factors

  

Value

 

Temperature (°C)

C

 

30

 

Reaction time (min)

D

 

65

Â