Table 1 Sequences of the primer paires used in this research
Target genes | Primers sequences (5ʹ to 3ʹ) | Anealing temperatures (°C) | Products length (bp) | References |
|---|---|---|---|---|
cagA | F-GATAACAGGCAAGCTTTTGA-3′ R-CTGCAAAAGATTGTTTGGCA-3′ | 50 | 349 | This study |
vacA | F-CAATCTGTCCAATCAAGCGAG-3′ R-GCGTCAAAATAATTCCAAGG-3′ | 58 | 567 | This study |
dupA | F-ACGATTGAGCGATGGGAATA-3′ R-AAGCTGAAGCRTTTGTAACGA-3′ | 53 | 467 | This study |
iceA1 | F-GTGTTTTTAACCAAAGTATC-3′ R-CTATAGCCASTYTCTTTGCA-3′ | 50 | 247 | This study |
iceA2 | F-GTTGGGTATATCACAATTTAT-3′ R-TTRCCCTATTTTCTAGTAGGT-3′ | 50 | 235 | This study |
oipA | F-GCTATATTMAAGGACAAGGYAG-3′ R-CCAGGAACAGA ACCAACR-3′ | 53 | 316 | This study |