Fig. 4

Function analysis of PEDV-S1S2J antibodies. a Determination of the antibody concentration for half-maximal binding (EC₅₀) with the S1S2J protein using ELISA analysis. To ascertain the binding affinities of these three antibodies to infected cells, Vero E6 cells were infected with PEDV-SP-C strain (G1 type) for 48 h. PEDV-infected cells were detected by PEDV S1S2J antibodies; b flow cytometry analysis; and c immunofluorescence assay