Skip to main content
AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Characterization of monoclonal antibodies against porcine epidemic diarrhea virus S1/S2 junction protein

Fig. 3

Epitope mapping of PEDV-S1S2J antibodies. a The full-length PEDV-S1S2J protein was truncated into three segments (designated as PEDV-S1S2J-N, PEDV-S1S2J-M and PEDV-S1S2J-C) with 15-amino acid overlap between neighboring parts. b These three truncated proteins were expressed in the Expi293 expression system, purified by a HiTrap Protein A HP affinity purification column and identified by Western blot using anti-mouse IgG antibody. c The purified truncated proteins were used as the coating antigen in ELISA analysis to detect the epitopes of 3-5G, 7-9D, and 8-3B mAbs

Back to article page