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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Characterization of monoclonal antibodies against porcine epidemic diarrhea virus S1/S2 junction protein

Fig. 1

Preparation of PEDV-S1S2J antibodies. a The recombinant vector pcDNA3.4ss-his-S1S2J-mFc containing the cytomegalovirus (CMV) enhancer region (CMV enhancer), CMV promoter, human interleukin-2 (IL-2) signal peptide, 10 × His, S1S2J gene fragment (630–800aa) of spike protein gene sequences from PEDV AJ1102, mouse IgG2a Fc gene (mFc), woodchuck hepatitis post-transcriptional regulatory element (WPRE) and HSV TK poly (A) was prepared for expression of recombinant PEDV-S1S2J protein in Expi293 cells. b The recombinant PEDV-S1S2J protein was purified by using a HisTrap HP column and analyzed by SDS-PAGE. BALB/c mice were immunized with recombinant PEDV-S1S2J protein 4 times. c The serum titers of the immunized mice were detected by ELISA analysis. Splenocytes prepared from immunized mice were fused with SP2/0 cell. d Hybridoma cells were screened by ELISA for the presence of antibodies against the S1S2J protein

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