Table 1 Different primers used in this study to detect different species of gut bacteria
Primer name | Primer Sequence | Annealing Temp. | Size bp | Reference | |
|---|---|---|---|---|---|
All bacteria | F | GAGTTTGATCCTGGCTCAG | 51 | 312 | (Ginige et al. 2013) |
R | GCTGCCTCCCGTAGGAGT | ||||
Porphyromonas gingivalis | F | AATCGTAACGGGCGACACAC | 53 | 594 | (Benkirane et al. 1995) |
R | GGGTTGCTCCTTCATCACAC | ||||
Fusobacterium spp. | F | GGATTTATTGGGCGTAAAGC | 51.5 | 162 | (Castellarin et al. 2012) |
R | GGCATTCCTACAAATATCTACGAA | ||||
E. coli | F | TGGGAGCGAAAATCCTG | 47.5 | 219 | (Maheux et al. 2009) |
R | CAGTACAGGTAGACTTCTG | ||||
Providencia spp. | F | ACCGCATAATCTCTTAGG | 43.5 | 514 | (Burns et al. 2015) |
R | CTACACATGGAATTCTAC | ||||
Bifidobacterium spp. | F | CTCCTGGAAACGGGTGG | 51 | 551 | (Matsuki et al. 2002) |
R | GGTGTTCTTCCCGATATCTACA | ||||
Prevotella intermedia | F | CGAACCGTCAAGCATAGGC | 54 | 368 | (Kim et al. 2011) |
R | AACAGCCGCTTTTAGAACACAA | ||||
Bacteroides spp. | F | AAGGGAGCGTAGATGGATGTTTA | 55 | 193 | (Huijsdens et al. 2002) |
R | CGAGCCTCAATGTCAGTTGC | ||||
Clostridium spp. | F | CGGTACCTGACTAAGAAGC | 50 | 429 | (Bartosch et al. 2004) |
R | AGTTTGATTCTTGCGAACG | ||||
Lactobacillus spp. | F | AGCAGTAGGGAATCTTCCA | 50 | 334 | (Collado et al. 2008) |
R | CACCGCTACACATGGAG | ||||