Deletion of Rap-phosphatases for quorum sensing control in Bacillus and its effect on surfactin production

AMB Express

Table 3 Overview of cultivation and process parameters up until a cultivation time of t = 48 h. Calculations were performed at P_max, if not indicated otherwise. Due to partially high standard deviations, additional technical replicates were performed for certain surfactin measurements and taken into account for the calculation of the mean values

Parameter	KM1016 168 sfp⁺		CT5 ΔoppA		CT10 ΔrapC		CT11 ΔrapF		CT12 ΔrapH
X _max [g/L]	8.3 ± 0.0	24 h	5.5 ± 0.0	24 h	10.9 ± 0.5	48 h	8.7 ± 0.0	48 h	8.6 ± 2.1	24 h
P _max [mg/L]	1245.4 ± 18.1	18 h	23.8 ± 0.7	12 h	3395.2 ± 29.8	24 h	3175.0 ± 24.0	24 h	1181.8 ± 245.0	24 h
P _srfA - *lacZ* _max [MU]	289.7 ± 13.0	18 h	n.d.	n.d.	346.2 ± 13.4	18 h	338.9 ± 11.6	18 h	553.8 ± 28.5	18 h
Y _P/X [g/g] at P _max	0.83		0.01		1.33		1.13		0.96*
q _max [g/(g·h)]	0.08		0.002		0.12		0.11		0.11
q _overall [g/(g·h)]	0.05		0.001		0.06		0.05		0.05*

* Calculation of process parameters for strain CT12 was performed at t = 18 h. Here, comparable surfactin levels (1133.4 ± 97.3 mg/L) were achieved, taking into account the standard deviation, which also occurred for the OD₆₀₀ measurement at t = 24 h (Figure S2)