Table 2 The RPA-Cas12a assay system
From: Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a
Component | Volume (µL) |
|---|---|
NEBuffer r2.1 (10 ×) | 2 |
Cas12a (1 µM) | 1 |
crRNA (1 µM) | 1.2 |
Fluorescent ssDNA reporter (2 µM), or Lateral flow ssDNA reporter (5 µM) | 1 |
RNase inhibitor RNase free water RPA products | 0.25 13.55 1 |