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Table 1 The RPA reaction system

From: Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a

Component

Volume (µL)

Primer F (10 µM)

2.4

Primer R (10 µM)

2.4

Primer-free rehydration buffer

29.5

RNase free water

11.2

Template

2

  1. Add the above reaction mixure to a tube containing lyophilized RPA enzyme. Then add 2.5 µL 280 mM MgOAc before incubation at 39 ℃ for 15–20 min
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