Fig. 3
From: Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a
Specificity and LoD of RPA-Cas12a system a, b Specificity of RPA-Cas12a-fluorescent assay, with reaction lasting for 30 min. c The LoD of RPA-Cas12a-fluorescent assay, with reaction lasting for 30 min. d Specificity of RPA-Cas12a-LFD assay, with reaction lasting for 30 min. e The LoD of RPA-Cas12a-LFD assay, with reaction lasting for 30 min. Data points represent at least four biologically independent experiments. Error bars indicate the mean ± SD. AU, arbitrary unit. The asterisk indicates a significant difference compare with the control of no targets by one-way ANOVA analysis (*P < 0.05, ****P < 0.0001). ns, not statistically significant. Ng, N. gonorrhoeae ATCC43069; Nm. 1, N. meningitidis. 1; Nm. 2, N. meningitidis. 2; Ns. 1, N. sicca. 1; Ns. 2, N. sicca. 2; Pae, P. aeruginosa