Fig. 2
From: Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a
Construction of the RPA-Cas12a-LFD assay a, b The optimization of the concentration of the lateral flow detection ssDNA reporter (3.125 nmol/L, 6.25 nmol/L, 12.5 nmol/L, 25 nmol/L, 62.5 nmol/L, 125 nmol/L, 250 nmol/L). C, control line; T, test line. Band intensity, intensity of the test line was measured by ImageJ. c The optimization of the ratio of Cas12a reaction mixture to Hybri Detect Assay Buffer. Cas12a reaction mixture: Hybri Detect Assay Buffer (1:1, 1:3)