Fig. 1
From: Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a
Optimizations of the RPA primer pairs and the CRISPR/Cas12a cleavage time durations. a Evaluation of the specificity of the RPA primer pair No.3 by electrophoresis on a 2% agarose gel. 1, N. gonorrhoeae ATCC43069; 2, E. faecium; 3, N. sicca; 4, N. sicca; 5, N. meningitidis; 6, N. meningitidis; 7, P. aeruginosa; 8, A. radioresistens; 9, E. cloacae; 10, K. pneumoniae. b Evaluation of the specificity of the RPA primer pair No.6 by electrophoresis on a 2% agarose gel. 1–7, N. gonorrhoeae clinical isolates; 8, A. radioresistens; 9, K. pneumoniae; 10, N. meningitidis; 11, N. meningitidis. BP, base pair; M, marker. c Evaluation of the specificity of the RPA primer pairs by Cas12a detection system. RPA reactions were performed with 6 sets of primer pairs. d Optimizations of the CRISPR/Cas12a cleavage time durations. Ng, N. gonorrhoeae ATCC43069 (1 ng/µL); Nm, N. meningitidis. AU, arbitrary unit