Fig. 3

Multiple amino acid sequence alignment comparing GH16B β-agarase with nine GH16 family β-agarases available in the Genbank database and their phylogenetic relationship. a Amino acids are designated using one-letter abbreviations after the alignment of aa sequences using Clustal X (Larkin et al. 2007). Identical residues in all sequences are indicated by asterisks under the column; conserved substitutions are indicated by colons, semiconserved substitutions are indicated by dots, and conserved residues are highlighted using Clustal X color scheme. Deletions are indicated by dashes. The location of N-terminal 22-aa signal sequence of GH16B β-agarase was predicted using SignalP version 5.0. b Phylogenetic relationships between GH16B β-agarase and nine related GH16B β-agarases based on their aa sequence similarities. The unrooted phylogenetic tree was constructed using UPGMA (Sneath and Sokal 1973). Numbers at nodes represent the levels of bootstrap support