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Table 2 a: Stability of Pull_Met in presence of some metal ions, β-mercaptoethanol, and EDTA

From: A molecular study on recombinant pullulanase type I from Metabacillus indicus

 

Residual activity (%)

Controla

100.00

Metal ion

(5 mM)

(10 mM)

MnSO4

150.53 ± 4.25

155.89 ± 8.97

NaCl

184.47 ± 5.61

134.71 ± 1.82

CaCl2

93.03 ± 2.20

97.64 ± 7.06

CuSO4

82.41 ± 5.90

71.28 ± 1.10

ZnCl2

74.09 ± 2.84

68.24 ± 0.12

KCl

140.05 ± 0.70

145.15 ± 5.04

FeSO4

79.00 ± 9.03

51.23 ± 0.54

Mg SO4

112.92 ± 9.40

92.25 ± 4.18

HgCl2

20.17 ± 1.25

28.04 ± 1.27

NiSO4

0.00

0.00

β-mercaptoethanol

173.79 ± 4.20

202.91 ± 5.61

EDTA

18.60 ± 0.61

23.78 ± 0.83

  1. Readings are the average of three readings ± SE.