Table 1 Purification table of Pull_Met using nickel-agarose affinity matrix
From: A molecular study on recombinant pullulanase type I from Metabacillus indicus
Purification step | Total Units | Total mg protein | Specific activity (U/mg) | Fold * | Recovery (%) |
|---|---|---|---|---|---|
Crude cell lysate | 915.00 | 43.50 | 21.03 | 1.0 | 100 |
After nickel affinity agarose chromatography column | 900.0 | 1.33 | 667.60 | 31.7 | 98.3 |