Fig. 6
From: A molecular study on recombinant pullulanase type I from Metabacillus indicus
Multiple sequence alignment between Pull_Met and other pullulanases type I from other species. A: the amino acid sequence of pullulanase type I signature motif highlighted by rectangle. B: the amino acid sequence of conserved region I in GH13 family highlighted by rectangle. C: the amino acid sequence of conserved region II in GH13 family highlighted by rectangle. D: the amino acid sequence of conserved region III in GH13 family highlighted by rectangle. E: the amino acid sequence of conserved region IV in GH13 family highlighted by rectangle. The accession number of pullulanases were AEW23439.1 (Anoxybacillus sp.), WP_004439017.1 (B. methanolicus), WP_251605681.1 (Priestia koreensis), UJT32179.1 (Priestia megaterium), UZX50359.1 (M. indicus), 2E8Y (AMyx protein of Bacillus cereus), WP_068759737 (Turicibacter sp.), WP_010899405.1 (Halakalibacterium halodurans), WP_011064811.1 (Oceanobacillus iheyensis), WP_008826932.1 (Haloplasma contractile), ACN58254.1 (Thermatoga neapolitana), WP_002277520.1 (Streptococcus mutans), WP_000283071.1 (Streptococcus pneumoniae), WP_067193616.1 (Strreptocccus sp.), and AVI10282.1 (Alkalihalophilus pseudofirmus). The catalytic triad residues: Asp, Glu, and Asp are indicated by asterisks